Ecological Response in the Integrated Process of Biostimulation and Bioaugmentation of Diesel-Contaminated Soil

The study applied microbial molecular biological techniques to show that 2.5% 3.0% (w/w) of diesel in the soil reduced types and number bacteria destroyed communities responsible for nitrogen cycle. In meantime, alkane degradation gene alkB polycyclic aromatic hydrocarbons (PAHs) nah evolved contaminated soil. We evaluated four different remediation procedures, which biostimulation-bioaugmentation joint process reached highest rate diesel, 59.6 ± 0.25% 27 days. Miseq sequencing quantitative polymerase chain reaction (qPCR) showed compared with uncontaminated soil, repaired provides abundant functional genes related cycle, most significant lifting effect on degrading γ-proteobacteria. Quantitative analysis shows can be colonized Gas chromatography-mass spectrometry (GC-MS) results components remaining after are alcohol, lipids a small amount fatty amine compounds, have very low toxicity plants. an on-site experiment, content decreased from 2.7% 0.3 1.12% 0.1 one month treatment. physical chemical properties returned normal levels, confirming practicability biosimulation-bioaugmentation jointed process.